Innovation Team: Sugarcane pest and disease management and cultivation
Main research areas:(1)pathogen-host interactionsinsugarcane;(2)green controlofsugarcanepests anddiseases;(3)highly efficient cultivation of sugarcane.
Chiefscientist:BaoshanChen, professor, PhD
Team members:RonghuiWen, professor, PhD; Ru Li, professor, PhD;Haiyan Wu Professor,PhD;JiaorongMeng, associate professor, PhD;DehongZheng associate professor, PhD;XipingYang associate professor, PhD; YangZhaoassociate professor, PhD;ChengwuZhou, associate professor, PhD;XiongbiaoXu,assistant professor, PhD; Shan Lu, assistant professor, PhD.
Team group photo
Achievements
Developmentof smut-resistant sugarcanevarieties.Threenewvarieties,ZZ1, ZZ6 and ZZ9 with extremely high resistance to smut and preferred agronomic traits were successfully bred.Smut rate for 3rdratoonyearwas kept under 0.5% in the field in large-scale production.
Characterization of mechanism ofsmutresistance in ZZ1.Thesmutresistance in ZZ1 wasidentified to beprimarily external resistance(Guo et al. 2024)andalkanolabundancewas implicated to be associated withthe smut resistance(Liu et al., 2022).Smutresistancewas mappedtoaYZ-Rec-Chr06A chromosome spanning approximately 33.35 Mb and containing 1902 genes.There is a significant presence ofresistance-relatedgenes, such as flagellin sensing 2 (FLS2), wall-associated kinase (WAK),andgenes related to cell wall formation, such as glucuronic acidxylanand hemicellulose synthesisin this region(Bao et al, 2024).
Identification of pathogenicity/virulence genes in the smut fungus.With the developmentof efficientmethods forgene knockoutin the smut fungusSporisouriumscitamineum(Lu at el., 2017)andefficient inoculation method for assay the virulence of the fungal strains or mutants (Guo et al., 2024),essentialvirulence-related genes ofS.scitamineumwere identified (Zhu et al., 2019;Lu et al., 2021;Lu et al., 2022;Zhang et al. 2023).It was also found that filamentous growth couldbe uncoupled with the virulence inS.scitamineum(Lu et al., 2021).
Sugarcane diseasesurveillance.Identification ofFusariunsaccharias the dominant pathogen for sugarcanePokkahboenginChina(Meng et al., 2019);first reportthatDickeyazeaecausedstalk bacterial soft rot of sugarcane(Yang et al., 2021);biological and phylogeneticcharacterization ofStagonosporatainanensis, thepathogencausingsugarcaneleafblightin China(Wang et al., 2023);survey ofsugarcane striate virus in Saccharum species in China(Lin et al., 2021); rapid viral disease diagnosis (Chen et al., 2019; Han et al. 2022a;Han et al. 2022b; Yao et al; 2020).
Selected publications
Zhou, Q.; Yao, Z.; Cao, X.; Chen, Y.; Zou, C.; Chen, B. Fusariumsaccharihypovirus1, a Member ofHypoviridaewith Virulence Attenuation Capacity in Phytopathogenic Fusarium Species. Viruses,2024, 16, 608. https://doi.org/ 10.3390/v16040608
Bao, Y., Zhang, Q., Huang, J., Zhang, S., Yao, W., Yu, Z., Deng, Z., Yu, J., Kong, W., Yu, X., Lu, S., Wang, Y., Li, R., Song, Y., Zou, C., Xu, Y., Liu, Z., Yu, F., Song, J., Huang, Y., Zhang, J., Wang, W. Chen, B, Zhang, X & Zhang, M.A chromosomal-scale genome assembly of modern cultivated hybrid sugarcane provides insights into origination and evolution. NatCommun.,2024,15, 3041 https://doi.org/10.1038/s41467-024-47390-6.
Guo F., Meng J., Huang J., Yang Y., Lu S.and Chen B.An efficient inoculation method to evaluate virulence differentiation of field strains of sugarcane smut fungus. Front. Microbiol.,2024,15:1355486.doi: 10.3389/fmicb.2024.1355486
Li, X., Liu, Z., Wu, H., YuZ, MengJ., ZhaoH., DengX.,SuY., ChenB.,and LiR.Four sugarcaneScDIRgenes contributetoligninbiosynthesisanddiseaseresistancetoSporisoriumscitamineum.PhytopatholRes 2024,6:17. https://doi.org/10.1186/s42483-024-00237-w
Chen, Y.; Yao, Z.; Zhao, L.; Yu, M.; Chen, B.; Zou, C. Redundant and Distinct Roles of Two 14-3-3 Proteins in Fusariumsacchari, Pathogen of SugarcanePokkahBoengDisease. J. Fungi 2024, 10:257. https://doi.org/10.3390/jof10040257
Liang, H.; Li, F.; Huang, Y.; Yu, Q.; Huang, Z.; Zeng, Q.; Chen, B.; Meng, J.FsCGBP, aCutinaseG-Box Binding Protein, Regulates the Growth, Development, and Virulence of Fusariumsacchari, the Pathogen of SugarcanePokkahBoengDisease. J. Fungi 2024, 10:246. https:// doi.org/10.3390/jof10040246
Xu X, Lou Y, Liang K, Liu J, Wang Z, Chen B, Li W. The P2 nucleic acid binding protein of Sugarcane bacilliform virus is a viral pathogenic factor. Peer J.2024;12:e16982,DOI 10.7717/peerj.16982
Zhao J, Li S, Xu Y, Ahmad N,KuangB, Feng M, Wei N, Yang X (2023). ThesubgenomeSaccharumspontaneumcontributes to sugar accumulation in sugarcane as revealed by full-length transcriptomic analysis. J Adv Res 54: 1-13.
Wang M, Qi X, Shi Y, Zhao J, Ahmad S, Akhtar K,Chen B, Lian T, He B, Wen R. Sugarcane straw returning is an approaching technique for the improvement of rhizosphere soil functionality, microbial community, and yield of different sugarcane cultivars. Front Microbiol.2023,14:1133973.
ZhangH., YangY., GuoF., ShenX., LuS., ChenB.(2023)SsRSS1 mediates salicylic acid tolerance and contributes to virulence in sugarcane smut fungus.Journal of Integrative Agriculture.22(7): 2126–2137,doi.org/10.1016/j.jia.2022.10.006
Huang, Z.; Shi, Q.; Zeng, Q.; Liang, H.; Yu, Q.; Meng, J.; Chen, B.Isolation, Characterization and Phylogenetic Analysis ofStagonosporatainanensis, the Pathogen Causing Sugarcane Leaf Blight in China. Agronomy 2023, 13, 1136. https:// doi.org/10.3390/agronomy13041136
Wang M, Qi X, Shi Y, Zhao J, Ahmad S, Akhtar K, Chen B, Lian T, He B and Wen R.Sugarcane straw returning is an approaching technique for the improvement of rhizosphere soil functionality, microbial community, and yield of different sugarcane cultivars. Front. Microbiol.2023,14:1133973.doi: 10.3389/fmicb.2023.1133973
Wang, Y.; Li, R.; Chen, B.S.Cytogenetic Characterization and Metabolomic Differences of Full‐Sib Progenies ofSaccharum spp. Plants,2023, 12, 810. https://doi.org/10.3390/ plants12040810
Liang KL, Liu JY, Bao YY, Wang ZY, Xu XB. Screening and Identification ofHost Factors Interacting with the Virulence Factor P0 Encoded by Sugarcane Yellow Leaf Virus by Yeast Two-Hybrid Assay. Genes (Basel).2023,14(7):1397.
XueY., ShiY., QiY., YuH., ZouC., CaoX., SunC., ChenB., WangH.Epigenetic and genetic contribution for expression bias of homologous alleles in polyploid sugarcane. Agronomy, 2022; 12, 2852;https://doi.org/10.3390/agronomy12112852
LiS,DuanW, Zhao J, Jing Y, Feng M,KuangB, Wei N, Chen B, Yang X (2022). Comparative Analysis of Chloroplast Genome in Saccharum spp. and Related Members of ‘Saccharum Complex’. Int. J. Mol. Sci. 23, 7661
Han, Z.; Yang, C.; Xiao, D.; Lin, Y.; Wen, R.; Chen, B.; He, X. A Rapid, Fluorescence Switch-On Biosensor for Early Diagnosis of Sorghum Mosaic Virus. Biosensors,2022, 12, 1034. https://doi.org/ 10.3390/bios12111034
Han, Z.; Du, P.; Wen, R.; Chen, B.; He, X.Signal “Off-On” Biosensor Based on Fluorescence Resonance Energy Transfer (FRET) for Detection of Sorghum Mosaic Virus. Analytica,2022, 3, 385–393. https://doi.org/10.3390/ analytica3040026
XueY, Zou C, Zhang C, Yu H, Chen B.and Wang H.Dynamic DNA methylation changes reveal tissue-specific gene expression in sugarcane. Front. Plant Sci.2022,13:1036764.doi: 10.3389/fpls.2022.1036764
Lu S., Zhang H., Guo F., Yang Y., Shen X.and Chen B.SsUbc2, a determinant of pathogenicity, functions as a key coordinator controlling global transcriptomic reprogramming during mating in sugarcane smut fungus. Front. Microbiol.2022,13:954767.doi: 10.3389/fmicb.2022.954767
Liu, Z.; Li, X.; Li, J.; Zhao, H.; Deng, X.;Su, Y.; Li, R.; Chen, B. Identification of Gene Modules and Hub Genes Associated withSporisoriumscitamineumInfection Using Weighted Gene Co-Expression Network Analysis. J. Fungi,2022, 8, 852. https://doi.org/10.3390/ jof8080852
Li,S.;Duan, W.; Zhao, J.; Jing, Y.; Feng, M.;Kuang, B.; Wei, N.; Chen, B.; Yang, X. Comparative Analysis of Chloroplast Genome in Saccharum spp. and Related Members of ‘Saccharum Complex’. Int. J. Mol. Sci. 2022, 23,7661. https://doi.org/ 10.3390/ijms23147661
Li, X.; Liu, Z.; Zhao, H.; Deng, X.;Su, Y.; Li, R.; Chen, B. Overexpression of SugarcaneScDIRGenes Enhances Drought Tolerance inNicotianabenthamiana.Int. J. Mol. Sci. 2022, 23, 5340. https://doi.org/10.3390/ijms23105340
Liu, Z.; Lan, X.; Li, X.; Zhao, H.; Gan, J.; Li, R.; Chen, B. A Plant-Derived Alkanol Induces Teliospore Germination inSporisoriumscitamineum. J. Fungi 2022, 8, 209.https://doi.org/ 10.3390/jof8020209
Lu, S.; Wang, Y.; Shen, X.; Guo, F.; Zhou, C.; Li, R.; Chen, B. SsPEP1, an Effector with Essential Cellular Functions in Sugarcane Smut Fungus. J. Fungi 2021, 7, 954. https:// doi.org/10.3390/jof7110954
LinY., AliN.,HajimoradM. R., ZhangL., QiX., ZhouL., WenRand ChenB.Incidence, geographical distribution and genetic diversity of sugarcane striate virus in Saccharum species in China.Plant Disease,2021,105:3531-3537, doi:10.1094/PDIS-10-20-2307-RE
Lu S, Guo F, Wang Z, Shen X, Deng Y, Meng J, Jiang Z, Chen B.Genetic Dissection of T-DNA Insertional Mutants Reveals Uncoupling of Dikaryotic Filamentation and Virulence in Sugarcane Smut Fungus. Phytopathology,2021,111:2303-2308,doi: 10.1094/PHYTO-03-21-0114-R
YangY.,YaoZ.,ZhangM.,ZouC.,ChenB.First report of stalk bacterial soft rot of sugarcane caused byDickeyazeaein China.Plant Dis.2021,105(4).doi: 10.1094/PDIS-10-20-2234-PDN.
Liu Y, Liu Q, Zhao X,XieS, Wang Z,Wen R, Zhang M.and Chen B.Effect oftwodifferentsugarcanecultivars onrhizospherebacterialcommunities ofsugarcane andsoybeanuponintercropping.Front. Microbiol.2021,11:596472.doi: 10.3389/fmicb.2020.596472
Yao Z., Zou C., Peng N., Zhu Y.,Bao Y., Zhou Q., Wu Q., Chen B.andZhang M.(2020)ViromeIdentificationand Characterization ofFusariumsacchariandF.andiyazi: CausativeAgents ofPokkahBoengDiseasein Sugarcane.Front. Microbiol. 11:240.doi: 10.3389/fmicb.2020.00240
ChenH.,AliN.,LvW.,ShenY.,QingZ.,LinY.,ChenB.,WenR.Comparison of IC-RT-PCR, Dot-ELISA and Indirect-ELISAfor the Detection of Sorghum Mosaic Virus in Field-GrownSugarcane Plants. Sugar Tech. 2020,22(1):122-129.https://doi.org/10.1007/s12355-019-00738-5
MengJ. R., HuangH. J., LiY. X., LiY. J., LiJ. Q., and ChenB.S.First Report ofFusariumsacchariCausing SugarcanePokkahBoengin China. PlantDis.2019,104(5),https://doi.org/10.1094/PDIS-05-19-0906-PDN.
ZhuG., DengY.,E.,MeixinYan, CuiG., WangZ., ZouC., ZhangB., XiP., ChangC., ChenB, JiangZ.Identification and Functional Analysis of thePheromone Response Factor GeneofSporisoriumscitamineum. Front. Microbiol.2019,10:2115.doi: 10.3389/fmicb.2019.02115
ChangC,Cai E,Deng Y, Dan M,QiuS, Chen B, Zhang L, Jiang Z. (2019)cAMP/PKA Signaling Pathway Regulates Redox homeostasis essential forSporisoriumscitamineumMating/Filamentation and Virulence.Environmental Microbiology. Environ Microbiol,21(3):959-971.Doi:10.1111/1462-2920.14496
LuS., ShenX., ChenB.Development ofan efficient vectorsystemfor gene knock-out and nearin-cis gene complementation in thesugarcane smut fungus. Scientific Reports,2017,7: 3113;DOI:10.1038/s41598-017-03233-7
