Jiuxiang Wang,Zhouxiang Liao,Xia Jin,Lindong Liao,Yaqi Zhang,Rongbo Zhang,Xiyao Zhao,Huajun Qin,Jianghong Chen,Yongqiang He,Chuxiong Zhuang,Jiliang Tang,Sheng Huang

The Plant Journal,12 July 2024 https://doi.org/10.1111/tpj.16929

Abstract

Bacterial leaf streak (BLS), caused byXanthomonas oryzaepv.oryzicola(Xoc), is a major bacterial disease in rice. Transcription activator-like effectors (TALEs) fromXanthomonascan induce host susceptibility (S) genes and facilitate infection. However, knowledge of the function ofXocTALEs in promoting bacterial virulence is limited. In this study, we demonstrated the importance of Tal10a for the full virulence ofXoc. Through computational prediction and gene expression analysis, we identified the hexokinase geneOsHXK5as a host target of Tal10a. Tal10a directly binds to the gene promoter region and activates the expression ofOsHXK5. CRISPR/Cas9-mediated gene editing in the effector binding element (EBE) ofOsHXK5significantly increases rice resistance toXoc, whileOsHXK5overexpression enhances the susceptibility of rice plants and impairs rice defense responses. Moreover, simultaneous editing of the promoters ofOsSULTR3;6andOsHXK5confers robust resistance toXocin rice. Taken together, our findings highlight the role of Tal10a in targetingOsHXK5to promote infection and suggest thatOsHXK5represents a potential target for engineering rice resistance toXoc.

原文链接：https://onlinelibrary.wiley.com/doi/10.1111/tpj.16929

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